IGF2BP2 Knockout cell line (HSC3)

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IGF2BP2 Knockout cell line (HSC3) Order

IGF2BP2 Knockout cell line (HSC3)
Catalog#
YKO-H100
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
IGF2BP2 Knockout cell line (HSC3)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
IGF2BP2 Knockout cell line (HSC3)
Cell Morphology
Endothelial cells, adherent
Passage ratio
1:3~1:5
Species
Human
Gene Name
IGF2BP2
Gene ID
10644
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Accession
CVCL_D9WC(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 4,Exon 5 (based on transcript IGF2BP2-202)
Validation: PCR+Sanger Sequencing
IGF2BP2 Knockout cell line (HSC3) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:HSC3		 STR Info (Cell bank)
Cell Line:HSC-3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11131113
D13S317 12121212
D7S820 13131313
D16S539 9999
VWA 14171417
TH01 69.369.3
AMEL XYXY
TPOX 8888
CSF1PO 11111111
D12S391 1818
FGA 2226
D2S1338 1923
D21S11 3031.2
D18S51 1515
D8S1179 1212
D3S1358 1717
D6S1043 1319
PENTAE 1115
D19S433 14.214.2
PENTAD 1010
D1S1656 1316
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the IGF2BP2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HSC3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, IGF2BP2 gene knockout IGF2BP2 Knockout cell line (HSC3) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
IGF2BP2 Knockout cell line (HSC3)
IGF2BP2 Knockout cell line (HSC3)
Catalog#
YKO-H100
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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